About the novel coronavirus (2019-nCoV) antigen test kit (colloidal gold method)

Views: 0     Author: Site Editor     Publish Time: 2022-03-24      Origin: Site

The 2019-nCoV antigen test is a novel test that allows rapid detection of unique protein fragments on or within the virus by using a swab to collect a sample from the oral or nasal cavity. So do you know what is the novel coronavirus (2019-nCoV) antigen test kit (colloidal gold method)? Let me tell you more about it!


Here is the content list:

  • Description

  • Principle

  • Meaning


Description

Novel coronavirus (2019-nCoV) antigen test kit (colloidal gold method) containing objects: sample extraction solution, test card, disposable sampling swab, sample collection tube.


Usage: first remove the disposable sampling stick from the paper-plastic bag, wipe the pharyngeal tonsils and posterior pharyngeal wall with the disposable sampling swab, then add 500u L of sample extraction solution (about 12 drops) vertically into the sample extraction tube, insert the collected swab into the solution in the sample extraction tube, rotate it 10 times near the inner wall of the tube so that the sample dissolves in the solution as much as possible, squeeze the swab tip along the inner wall of the extraction tube, in order to make The liquid remains in the tube as much as possible, remove and discard the swab, close the cap, equilibrate the test sample and the kit to room temperature, remove the test card from the foil pouch and place it horizontally on a dry surface, add 50 μ L (about 1 drop) of the treated sample to the sample well of the test card, read the displayed results in the observation area within 15 - 30 minutes, if the results appear only after 30 minutes, the results are invalid.


Interpretation of test results: If positive (+), there are 2 lines in the observation zone, one of which is the test line (T) and one of which is the quality control line (C). If it is negative (-), only one line appears in the observation area, and the line is the quality control line (C). The invalid result is that no ribbon appears on the quality control line (C), and regardless of whether the test line (T) shows a ribbon or not.


Principle

Novel coronavirus (2019-nCoV) antigen test kit (colloidal gold method),The N protein of the new coronavirus can be used as an immunogen to stimulate plasma cells to produce specific antibodies after the virus infects the body. Based on the double antibody sandwich ELISA principle, the sample is added dropwise to the sample pad and passed sequentially through the binding pad by liquid-phase chromatography, the detection line (T line), and the quality control line (Cline) on the NC membrane. The binding pad contains labeled antigen-specific antibodies that bind to the antigen (viral protein) in the sample. When the flow reaches the detection line (T-line), a second antigen-specific antibody immobilized on this line binds to the antigen again and will give a positive result. The quality control line (Cline) is coated with IgY antibodies that bind antibodies in the sample pad and is used to determine if the chromatography process is going well.


Significance

The Novel coronavirus (2019-nCoV) antigen test kit (colloidal gold method) can directly detect the presence of novel coronaviruses in human samples with fast and accurate diagnosis and low requirements for equipment and personnel. The use of a dual antibody sandwich method, which uses two antigen-specific antibodies to recognize and bind different epitopes of a target antigen, greatly reduces the chance of cross-reactivity and thus effectively improves its specificity. The antigen assay is cheaper, easy to produce, and very fast, making it suitable for the widespread detection of new coronaviruses.


With the above introduction, do you know a little more about the Novel coronavirus (2019-nCoV) antigen test kit (colloidal gold method)? I hope this small introduction will be helpful to you!


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