Publish Time: 2022-04-11 Origin: Site
When the human body is infected with a virus, immune cells are stimulated to produce specific antibodies. The two main types of specific antibodies produced include IgM antibodies and IgG antibodies. The former is produced in the initial immune response and is produced quickly and for a short period, while the latter is produced in the secondary immune response and is produced late and for a long period. Rapid immunoassay tests are mostly performed on test strips, and most of them apply the colloidal gold principle of antigenic antibodies. Let me introduce the novel coronavirus (SARS-COV-2) lgG/IgM antibody test kit (colloidal gold method) in detail next!
Here is the content list:
Principle
Examples
Based on the principle that the human immune system produces specific antibodies after infection with the new coronavirus, the virus-specific IgM and IgG antibodies in human serum are quickly and accurately checked by constructing plasmids expressing the N and S proteins of the new coronavirus as antigens and establishing the colloidal gold method and chemiluminescence method. novel coronavirus (SARS-COV-2) lgG/IgM antibody test kit (colloidal gold method) will effectively reduce the technical difficulty of laboratory detection of novel coronavirus pneumonia and greatly improve the efficiency of detection, especially suitable for screening a large volume of samples.
Our novel coronavirus (SARS-COV-2) lgG/IgM antibody test kit (colloidal gold method) contains items such as test strips. The test paper tests are divided into two types: antigen detection test paper and antibody detection test paper, mainly using the immunochromatographic platform. Taking the principle of antigen detection test paper as an example, the order of each part of the test paper is "sample pad - binding pad - NC membrane - absorbent pad". The sample pad is used for dripping the sample to be tested, and the sample will move toward the absorbent pad; the binding pad contains a complex of colloidal gold and IgM/IgG antibodies, and the antigen in the sample will be specifically bound by this complex and continue to move toward the absorbent pad; the NC membrane has two parts: detection line and quality control line, and the detection line is fixed with antigen-pairing antibodies, which can be used with the "colloidal gold-IgG antibody" moving to this part. The detection line is fixed with antigen-pairing antibodies, which can bind specifically to the "colloidal gold-IgM/IgG antibody-antigen" complex moving there and develop color to reflect whether the sample contains the antigen to be tested, while the quality control line contains secondary antibodies that can bind specifically to the "colloidal gold-IgM/IgG antibody" complex and develop color. The secondary antibody is used to test the quality of the test paper. In the same way, the antibody test strips replace only the specific antibodies used in each part for identification. The antigen-antibody immunoassay has significant advantages, including the fact that the test takes less than twenty minutes and does not involve complex and sophisticated instruments, but its limitations are also significant. The specificity and sensitivity of the antigen-antibody test are limited by the specificity of the antibody-antigen applied in the test, and it is not suitable for screening such patients because of the long incubation period of the virus and the fact that few antibodies are produced in the patient's body during the incubation and early stages of the disease. The antibody test is susceptible to interference by other substances in the body, and multiple tests must be taken. Therefore, this test is more likely to be used for preliminary screening of large groups of people to be tested but is not suitable as the main basis for confirming the diagnosis.
Through the above introduction, do you know a little more about the novel coronavirus (SARS-COV-2) lgG/IgM antibody test kit (colloidal gold method)? I hope this small introduction can be helpful to you!